Combatting Oxygen Shortages

April 23rd, 2010

Animal kingdom emerged on our planet when the atmosphere was still very poor in oxygen. It is no wonder that living organisms had to adapt themselves to an environment where oxygen was in short supply. However, we usually fail to notice another much more puzzling phenom­enon, namely, that animals living in the presence of exces­sive oxygen have managed to restrain the intensity of the oxidation processes taking place in their bodies as if they were always ready to extinguish a constantly threatening fire.

The amount of environmental oxygen is constant, and, if it does alter, it decreases. This explains why animals have different means of combatting oxygen shortages, but no means of protection against excess oxygen.

Paul Bert was the first to discover that breathing pure oxygen can be poisonous around a hundred years ago. This was such an unexpected discovery that scientists did not believe him and a suspicion arose that the oxygen used by Bert contained various poisonous admixtures. The experiments were repeated many times, but no matter how thoroughly the oxygen was purified, the animals which breathed it for prolonged periods inevitably perished.

Oxygen, Energy and Wastefulness

April 23rd, 2010

The question arises why living organisms use atmospheric oxygen if energy can be obtained by mere fermentation. There are many important reasons for this. Fermentation never results in the complete oxidation of a substance and, therefore, little energy is released. If one gram-molecule of glucose is completely oxidized to carbon dioxide and water, 673 large calories will be obtained. But with fermentation, which results in the formation of ethyl alcohol and carbon dioxide, only as little as 25 large calories will be released, i. e. almost 27 times less. This means that anaerobes have to use 27 times as much glucose as aerobes to obtain the same amount of energy. The difference is, of course, appre­ciable and nature cannot tolerate such wastefulness.

Another important reason is that substances such as ethyl and butyl alcohol, lactic and butyric acid, acetone, etc., which are bad for the organism, are formed as a result of fermentation. It is not easy to dispose of these harmful substances.

Respiration frequently produces combustible gases. Micro­organisms often release hydrogen. This is how microbes living in the intestine of termites breathe. Of the multicellular creatures, the larvae of some flies, in particular, release a great deal of hydrogen. Some organisms liberate not only hydrogen, but also methane and other gases, some of which are still not known, including spontaneously inflammable gases. It is a particularly beautiful sight when the gases, which have collected in the silt at the bottom of a pool, rise to the surface of the water and burn with a mysterious bluish flame.

How then have animals managed to change their way of breathing to such an extent and adapt themselves to an absence of oxygen? This did not prove difficult. At the dawn of life on the Earth there was little free oxygen and the earliest living creatures had to become anaerobes. It was not until the atmosphere became rich in oxygen that animals learned to burn energy-forming products completely. At the same time, the anaerobic method of breathing did not disappear but was passed on and finally came down to us.

As has been mentioned at the beginning, in all animals without exception the first stages of energy release proceed without oxygen. When aerobic animals felt like returning to the places where no oxygen could be obtained, they again had to restrict themselves to partial utilization of the energy contained in nutrient substances. To do this they had to remember how to render partially oxidized products harmless.

Methods of Oxidation

April 23rd, 2010

Oxidation by abstraction of hydrogen is termed fermen­tation; it results in the splitting of organic substances to form oxidized and reduced products and the liberation of the energy required by the organism.

The best known form of fermentation found in unicel­lular organisms is the breakdown of a glucose molecule into two molecules of ethyl alcohol (the reduced substance) and two molecules of carbon dioxide (the oxidized substance).

In multicellular organisms, the most common form of fermentation is lactic fermentation which involves the decom­position of carbohydrates, as, for instance, when a sugar molecule breaks down into two molecules of lactic acid which have less energy than the initial substance. The breakdown of carbohydrates is a gradual process consisting of a series of reactions. As a result, the oxygen in the molecule of sugar near to the inner carbon atom is trans­ferred to the external carbon atom. Energy is thereby liberated.

There is also another method of oxidation, that of electron loss, but whether it can be used by living organisms has not been adequately studied.